Journal: Mediators of Inflammation

Article Title: Interleukin-29 Enhances Synovial Inflammation and Cartilage Degradation in Osteoarthritis

doi: 10.1155/2016/9631510

Figure Lengend Snippet: The expression and cellular distribution of IL-29 and IL-28R α in the synovial tissues. IL-29 and IL-28R α in synovial tissues of OA patients ( n = 5) and HC ( n = 3) were detected by immunostaining (a) and semiquantification (b). Values in (b) are the mean ± SEM. ∗∗ P < 0.01 versus medium control. (c) Colocalization of IL-29 with CD68 or FGF-2 in OA synovium was detected by double immunofluorescence staining, and nuclei were counterstained with DAPI. All of the magnification in this figure was ×400.

Article Snippet: Recombinant human IL-29 and IL-1 β were purchased from Peprotech (Rocky Hill, NJ, USA); rabbit anti-human IL-29/IL-28R α polyclonal antibody and mouse anti-human CD68 monoclonal antibody were purchased from Abcam (Cambridge, MA, USA); mouse anti-human fibroblast growth factor-basic (FGF-2) monoclonal antibody was purchased from Millipore (Billerica, MA, USA); PE anti-human IL-28R α was purchased from BioLegend (San Diego, CA, USA); donkey anti-rabbit IgG-R and goat anti-rabbit IgG/TRITC were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA); DyLight TM 488-conjugated AffiniPure donkey anti-mouse IgG, peroxidase-conjugated sheep anti-rabbit secondary antibody, and peroxidase-conjugated sheep anti-mouse secondary antibody were purchased from Jackson Immunoresearch (West Grove, PA, USA); human IL-29 antibody was purchased from R&D Systems (Minneapolis, MN, USA); human IL-29 enzyme-linked immunosorbent assay (ELISA) reagent kits were purchased from Adlitteram Diagnostic Laboratories (San Diego, CA, USA); MMP-3 ELISA kit was purchased from USCN Life Science Inc. (Wuhan, China); Safranin O staining kit was purchased from ScienCell Research Laboratories (Carlsbad, CA, USA); primerScript TM RT reagent kit was purchased from TaKaRa (Dalian, China); power SYBR Green PCR Master Mix was purchased from Applied Biosystems (Carlsbad, CA, USA); phospho-STAT antibody sampler kit, phospho-c-Jun N-terminal kinases (JNK), phosphoextracellular signal-regulated kinases (ERK), phospho-p38, phospho-AKT, phospho-p65, phospho-I κκ B, and β -actin antibody were purchased from Cell Signaling Technology (Beverly, MA, USA); tissue culture reagents including Dulbecco's modified Eagle's medium (DMEM) and fetal bovine serum (FBS) were purchased from Gibco (Carlsbad, CA, USA).

Techniques: Expressing, Immunostaining, Control, Double Immunofluorescence Staining